Background: From the prospective study published in 2008, in which was reported the frequency of variants of HPV16 in invasive squamous cell carcinoma of cervix of Colombian population, we studied retrospectively one case in which was identified presence of the Asian-American variant of subclass c (AAc). A 43-year old woman was diagnosed with a high-grade squamous intraepithelial lesion of uterine cervix (HGSIL) in 1986. There were several colposcopy and directed biopsies with changes consistent with HGSIL and HPV infection. The gynecologist meeting decided that patient should be subjected to extended abdominal hysterectomy, being referred to another hospital. She returned to the National Cancer Institute on 2002 with a tumor of size of 5 cms, and is diagnosed with cervical cancer IIIB. She begins exclusive radiotherapy but abandoned and died in 2005. Aim: Make a diagnosis and molecular monitoring in biopsies of patient with pre-neoplastic lesions and cervical cancer Methods: Analysis of molecular markers was performed on biopsies taken in 1986 and 2002. Results: HPV16 E6 E-r and AAc mixed variants were detected in samples took in 1986. A HPV16 E-r and AAc variant was detected in 2002, which indicate persistence of the infection. Polymorphism analysis of Arg72Pro p53 in 1986 and 2002 showed an Arg/Pro genotype. An increment of expression of IGF1R, Survivin, GLUT1, and CAIX was observed in biopsies-(2002) compared with the analysis done 1986; hTERT expression in pre-neoplastic lesions was detected at 100%. Conclusions: In the prevention of cervical cancer, the presence of the AAc-HPV16 could be used as a prognostic marker of persistent infection, progression and treatment, as well hTERT expression as tumor progression marker. The analysis of molecular profiles that include biomarkers prognostic, predictive and molecular targets, and employed in this work could ensure early diagnosis and better therapeutic management of cervical lesions and cervical cancer.